Nacl on Peroxidase Activity

NaCl Effects on Peroxidase Activity My experiment was to see if adding NaCl to solution would have any effects on peroxidase activity. The materials that were used in this experiment were pH 7 buffer(DI water), peroxidase, NaCl, guaiacol and hydrogen peroxide; added in that order. Blanks were created for each NaCl concentration, 0%, 5%, 7. 5% and 10%. Each cuvette had . 5ml of pH 7 buffer, 1ml of peroxidase, . 02ml guaiacol for the experimental cuvettes and 0ml of guaiacol for the blank cuvettes, . 2ml of hydrogen peroxide and . ml of different concentrated NaCl in each cuvette. When it came to recording data for my experiment, I placed the cuvette in the spectrometer, which was set to 500nm, after adding the guaiacol and hydrogen peroxide right before. I recorded the absorbance every 15 seconds for 3 minutes. I ran the experiment twice for precision and got the average of the two tests. I then entered the data into JMP and made two graphs; one to see the relationship between the con centration of NaCl and activity and the other to see how salt affect enzyme activity.The first graph only required the time and the mean absorbance. To make the second graph, I had to find Time X, the time where reaction starts to slow down, which in my case was 90 seconds. The axes for the graph were Time X as the y-axis and NaCl concentrations as the x-axis. The graphs showed me that there is a relationship between NaCl concentrations and peroxidase activity. More the concentration of NaCl, the faster the reaction occurs.

Essay Example | Topics and Well Written Essays - 500 words - 107

Essay Example That is until one day when his desire uncoiled when he was offered a summer building job to carry out â€Å"real work†. He was willing to perform it precisely, when he reaches at the construction site, with no fear at all. It is clear that Rodriguez was not seeking for a summer job, rather a chance to work with his back and hands. His desire is to feel his body in a unique or new way; he wanted to know the meaning of real work, even if it lasted for a short time so long as he worked like hard for once. Just like men whose desire is to fulfill their sexual fantasies by performing in the City of Night, Rodriguez wanted to fulfill his erotic dream by trying to side with the working class, a task for himself and for his audience (Dunbar-Odom 37). As a simple laborer, Rodriguez had a positive attitude towards his contract. The work seemed simpler than his friends did and he had thought. He enjoyed doing it, since he says that he had many physical pleasures during the labor. Each day counted for him, as he woke up every morning with a new working spirit. During the day, his desire to work conquered all the barriers that came his way. Sometimes he could even do too much that his colleagues were amazed, but all was for the sake of fulfilling his fantasy (Rodriguez 275). During his shoveling work, he realized that he was not doing what was right. He was fooling himself by expecting to be admitted in the world of the laborer. He says, â€Å"I could not learn in three months what my father had meant by â€Å"real work,† to mean that what he had been doing up to that level was not sufficient enough to measure to what his father could recognize as hard work. He could not be pleased by the achievement of his son so far. He needed more experience and efforts and that for him to accomplish â€Å"real work,† he had to take quality time. According to his mother and father, â€Å"real work† means struggling with self-confidence to achieve

Trust Law Essay Example | Topics and Well Written Essays - 1000 words

Trust Law - Essay Example The reason that a review of the role and contribution of the law of trusts to the law relating to pensions is important is because in 2004 alone there were 927 billion worth of private pension funds under management in the UK, holding 16 per cent of the domestic ordinary share market, which is the largest in Europe and third largest in the world (Slattery and Nellis, 2004).Today pension funds of several British Corporations run into larger amounts athat their own market capitalisation (Slattery and Nellis, 2004). The law relating to pension schemes is derived from the old trusts law(sometimes even dating back to the eighteenth century). Cooper, D.R. (2000) . Even though the courts have recognised the different nature of pensions as compared to traditional trusts it seems that there are still gaps in this area.(Hales, C., Gough, O 2003) Private sector pension schemes are set up as trusts and a trust fund will be created to which the employer has to contribute(sometimes the employees may be required to contribute as well). The concept of trust developed with the purpose of the facilitation of the passage of inheritance and property to a trusted party (trustee or trustees) for the benefit of a third party (beneficiary or beneficiaries).Therefore when trusts are set up in the form of pensions for employee benefits the current and former employees along with their family members become the beneficiaries of this scheme. Hales, C., Gough, O. (2003) These schemes allow the trustees to own the legal title to the assets of the scheme and invest this .They must invest this capital for the benefits of the members of the scheme and look after the interests of pensioners and the current employees. Hales, C., Gough, O. (2003) Best interests have been defined as those confined to monetary goodwill i.e. being able to obtain maximum amount of pension through the prudent investment of the trustees. In this regard the Pensions Act 1995 puts an obligation upon the trustees to make prudent and risk free investment decisions. The Act prevents them from escaping liability through exclusion clauses in the deeds and must take care in ensuring that a proper person is appointed for the purposes of managing such investment.The trustees will be able to draw up a statement of investment principles.They will be able to decide upon whether a member leaves the scheme and whether to pay a certain person earlier due to death or ill health. The most significant intertwining of the law of trusts and law of pensions occurs when the Courts refuse to intervene into the trust matters following the extensive powers conferred upon the trustees to make decisions. (Duncan, C., Loretto, W., White, P.2001).However the Court will be prepared to intervene to ensure whether these powers have

Global Operations Management Essay Example | Topics and Well Written Essays - 250 words - 1

Global Operations Management - Essay Example 2) The organization identity must be aligned with the business structure the company chooses to implement. The expansion project can be set up using different managerial structures including centralized, decentralized, and matrix structures. Assuming that the company wants to start small with an emphasis on a specific project my recommendation is for the firm to use a matrix organization structure. â€Å"Matrix organizations are often set up to make organizations more flexible, to break down the old functions or geographic â€Å"silos† and encourage more cooperation across the business† (Global-integration, 2012). 3) There are plenty of legal factors that must be considered when expanding to a foreign country. The firm has to evaluate the environmental laws of the country. Secondly the company must study and carefully analyze the labor laws. A good strategy is to hire a human resource manager from the native country. The tax code of the country must be followed to maintain good relations with the government. A fourth legal consideration is local laws relating to the relationship between a manufacturer and local agents or distributors (Kentuckianabusinessforum, 2012). Kentuckianabusinessforum.com (2012). Legal Considerations in Operating in Foreign Markets. Retrieved February 17, 2012 from

Written Evaluation of a Patient Education Tool for an adult with an Essay

Written Evaluation of a Patient Education Tool for an adult with an acute or chronic alteration in health status - Essay Example There are, however, many applicable tools for patient education and the characteristics of each tool together with its advantages and disadvantages and the nature of the target patient or patient population are instrumental in determining suitability of a patient education tool. A patient’s age or literacy level, for example, determines effectiveness of a tool towards awareness creation. The aim of this paper is to evaluate a handout used for patient education for an adult with asthma. Introduction of the tool: Handout A hand out refers to a written material, in a print format, which is offered to an audience free of charge. It aims at communicating purposeful information to the audience and is therefore audience specific. It is a commonly applied tool in patient education and is distributed at strategic points in health care facilities. Handouts are majorly intended to create awareness, among patients, on preventive and management strategies for eliminating diseases or managi ng their impacts and the management aspect is very instrumental to adult patients with chronic diseases because their conditions requires effective, and for efficiency, self-reliant managerial approaches. The document, ‘Asthma’s impacts on the nation: Data from the CDC national asthma control program,’ is an example of handout that facilitates awareness on asthma, a chronic disease. The handout is authored by the Centers for Disease Control and Prevention, a national agency in the department of health in the USA. It is available in the internet but can easily be accessed in print form and disseminated to target audience such as attendants to a health care’s department of chronic diseases or in consultation rooms for asthma patients (IHS, n.d.; Centers for Disease Control and Prevention, n.d.). The tool’s design incorporates graphics with colors and pictures that facilitate its effectiveness by drawing and retaining audience’s attention. It fu rther identifies major points by isolating them in side boxes and using numerical and imagery representations to distinguish them. The handout also represents information in summarized point forms and this identifies clarity towards effective communication of the intended message. It is also relatively brief and is organized into headings. While it’s short length avoids monotony and therefore allows for audience concentration in reading the entire document, organization of information by titles that are further printed in bold identifies the tool’s content and therefore facilitate the intended educational objective for each section. The handout’s level of precision also identifies its efficiency because the bulleted points are conspicuous. The tool’s content defines asthma, and identifies symptoms, known causes, and its significance in the society. It further explores strategies for preventing and managing asthmatic conditions and risk factors. The handou t further explores the scope of asthma in the society by examining level of awareness and active participation in management initiatives among children and adults (Centers for Disease Control and Prevention, n.d.). Intended Audience The tool is appropriate for the adult population that is literate and is rational. Even though it is specific on a type of disease, asthma, the handout is appropriate for the entire literate population because of its significance to both those who are suffering from the chronic disease, and those who are affected by the disease. The affected population includes family members of those who suffer from this disorder and they may derive sufficient knowledge for helping asthma patients to manage their conditions effectively. The same significance applies to friends and even the society and allows for assistance

There is an ongoing debate on the use of capital punishment. Discuss Essay - 1

There is an ongoing debate on the use of capital punishment. Discuss the social, moral and historical aspects of this controversial topic - Essay Example religious leaders, and foreign press make quite a spectacle of how the Throughout the world legal systems regularly sentences people to die for the crimes they have committed. As such, many nations around the globe view the death penalty as something of a novelty (Debrevnik, 2004). From a purely economic standpoint, capital punishment is an extraordinarily expensive form of punishment. As compared with life in prison, the average cost of execution is approximately the same cost to keep a prisoner housed and fed for over 100 years (Petersen & Lynch, 2012). Currently, the average cost of execution in California exceeds 4 million dollars per criminal executed. Comparatively, the average cost to keep a prisoner housed and fed as well as ensuring proper health care and medicine usually does not exceed $35,000 per year (Semeshenko et al, 2012). At such an exorbitantly high expense, it is clear that choosing capital punishment on the grounds that killing the criminal will somehow save the state money over time is entirely illogical. As such, the argument for execution does not hinge on economic savings; instead, it hinges upon the Judeo-Christian belief of â€Å"an eye for an eye and a tooth for a tooth†. In this way, the major supporters of the death penalty believe that certain crimes are so heinous that rehabilitation is not possible. As such, one’s life is forfeit for certain crimes if convicted. This further raises the tangential issue of the purpose of prison; whether it is it to rehabilitate or to punish. According to death penalty activists, prison’s primary objective is to punish with rehabilitation being a very distant second. A secondary issue with the death penalty in its current form is that they death penalty has been proven not to be a significant deterrent against the crimes it punishes. It is obvious that murder rates throughout the world are comparably higher than almost any other nation that currently does not have the death penalty as

RPOGRAM ANALYSIS OF FIRE Essay Example | Topics and Well Written Essays - 250 words

RPOGRAM ANALYSIS OF FIRE - Essay Example The proposed gym and training area/center will be constructed such that 30% and 70% of it is indoors and outdoors respectively. In terms of design and planning, the program will resemble a military training program from which fire fighters can adapt the same training methods such as rope climbing and tacking hurdles. The application of the site analysis to the community program is to facilitate evaluation of developmental constrains. For instance, while the analysis of surrounding buildings and structures is essential, the topographical characteristics such as terrain are considered. Based on the weather, soil, and available vegetation, the design and construction of the gym and training area/center would rely on this information. From a business perspective, the site analysis will consider how different other variables such as demographics and distribution interact within the area. For instance, the program will make use of a central location from which prospective clients can access the premises. In addition, the dimensions of the gym and the training center will depend on the approximate number of clients within the community. Finally, the site analysis will consider legal aspects of architectural development such as building policies, regulation of private and public structures, and availabi lity space for rental or purchase (Zimmerman,